This research studied the quantity of synthetic colour in fruit and vegetable pickled that obtained from Saphandam, Srinakhon ,and Bonkai market and Rajabhat Nakhon Sawan University area by High Performance Liquid Chromatography (HPLC) with C-18 column using UV – VIS as a detector . The result to studied the suitable condition of HPLC used Ammonium Acetate buffer pH5 and Methanol(70:30) is mobile phase, flow rate 0.6 ml/min, temperature 30 ºC and detection was made at 430 nm respectively. The samples were extracted by Solid Phase Extraction (octadecylsilane cartridge:SPE C18 cartridge). The result adaptation SPE C18 cartridge by methanol, Ammonium acetate buffer. The samples were eluted by methanol and then measured quantity of synthetic colour by HPLC.