The purpose of this study was to investigate the efficiency of vitrification for thalassemic mouse embryos. There were two genotypes of thalassemic embryos 654 and BKO, produce by mating of wild type female (C57BL/6) and thalassemic male. Morphologically normal 2-cell embryos were selected for control and vitrification. Both embryos genotypes were vitrified in straw using 35% ethylene glycol, then stored in liquid nitrogen at least 7 days. After warming, vitrified embryos were cultured in M16 medium and blastocyst formation were evaluated. There was no different in blastocyst rates between vitrified and control group in both genotypes. Results showed that thalassemic mouse embryos can be cryopreserved by vitrification which is a good alternative for saving in maintenance cost of valuable animal model.