To identify key transporters involved in cadmium transport systems, we screened the yeast deletion mutants lacking transporters in rich medium containing 80 μM CdCl2. The screening revealed that 73 and 54 deletion mutants were sensitive and resistant, respectively, to cadmium. A large number of these mutants lacked ion transporter localized to plasma membrane. To investigate the role of these transporters in cadmium uptake, we then measured cadmium accumulations in these mutants by flame atomic absorption spectrometry. Our results suggested that, in addition to Zrt1p that is known to function as cadmium transporter, Fet4p, Ctr1p, and Fps1p may also be involved in influx of cadmium.