This research aimed to generate ABTS radical by the oxidation reaction of laccase enzyme.This method was easy, quick and high yield. These ABTS radicals were used for antioxidant activity assay of 31 local vegetables. The water extracts of fresh vegetables showed as high TEAC values as in Syzygium gratum (Wight)S.N. Mitra var. gratum, Cassia siamea Britt. and Azadirachta siamensis. The ethanol extract of dry vegetables showed in Syzygium gratum (Wight) S.N. Mitra var. gratum, Heliotropium indicum L.and Shrebera swieteniodes Roxb. It was found that the TEAC values determined using ABTS and DPPH radical were strongly consistency. Moreover, antioxidant activity also correlated with total phenolic content in the samples.